Journal: Cancer Science

Article Title: Mesenchymal-transitioned cancer cells instigate the invasion of epithelial cancer cells through secretion of WNT3 and WNT5B

doi: 10.1111/cas.12336

Figure Lengend Snippet: Cell–cell contact independent induction of secondary epithelial-to-mesenchymal transition (EMT) phenotype and invasiveness in neighboring epithelial cancer cells by mesenchymal-transitioned cancer cells. (a, b) A549 cells were co-cultured directly (a) or separately in transwell cell culture chamber (b) at the indicated cell numbers for 24 h and EMT-related protein expression were determined by western blotting. No cells (−), E-cells (E) or M-cells (M) were seeded in upper compartment of transwell chamber. In the separated co-culture, the total protein from E-cells in lower compartment was examined. (c, d) Epithelial A549 cells were treated with conditioned mediums (CM) from E-/M-A549 cells (c) or E-/M-Panc cells (d) for 48 h and subjected to Matrigel invasion assay or western blotting. Total invaded cells were counted after H&E staining. Data represented as the mean ± SD of triplicate experiment. ** P < 0.01 versus E-CM group by two-tailed Student's t test.

Article Snippet: For the direct co-culture experiment, E-cells and M-cells were re-seeded into 60 mm culture dish according to the indicated cell number and co-cultured for 24 h. For the separated co-culture experiment, 1 × 10 5 E-cells and 3 × 10 5 cells of M-cells were seeded into lower or upper compartments of the transwell chamber with 1 μm pore diameter (BD Falcon, Bedford, MA, USA) for 24 h.

Techniques: Cell Culture, Expressing, Western Blot, Co-Culture Assay, Invasion Assay, Staining, Two Tailed Test

Journal: Cell Death & Disease

Article Title: A novel function of IMPA2 , plays a tumor-promoting role in cervical cancer

doi: 10.1038/s41419-020-2507-z

Figure Lengend Snippet: a Cell migration of IMPA2 silencing and overexpressing SiHa or Hela cells and in the respective control cells was analyzed without Matrigel using transwell chamber. b Cell migration was analyzed by a wound healing assay. IMPA2 silencing and overexpressing SiHa or Hela cells were seeded and grown to full confluence. In total, 48 h after creating the wound, the wounding space was observed and photographed. The data represent the mean ± SDs of three replicates. ** P < 0.01; *** P < 0.001.

Article Snippet: Details of transwell migration assay was described previously , 2 × 10 4 cells in 200 µl of serum-free medium were added to the top chamber of the transwell (8-μm pore size, BD Biosciences, New Jersey, USA).

Techniques: Migration, Wound Healing Assay

Journal: Cell Death & Disease

Article Title: A novel function of IMPA2 , plays a tumor-promoting role in cervical cancer

doi: 10.1038/s41419-020-2507-z

Figure Lengend Snippet: a The expression levels of IMPA2 and MAPK signaling molecules ERK, p-ERK, p38, p-p38, JNK, p-JNK, and GAPDH protein in IMPA2 silencing SiHa or HeLa cells were detected by western blotting. b ERK and p-ERK expression of the inxenografts were detected by immunohistochemical staining and the immunoreactivity scores of t-ERK and p-ERK were shown. Scale bar, 50 μm. The migratory and proliferation potential of IMPA2 silencing SiHa or HeLa cells treated with FR 180204 or DMSO were analyzed by the transwell cell migration and colony formation assay ( c ). CCK-8 assay ( d ), wound healing assay ( e ). Number of migratory cells ( f ) and clone ( g ) was shown as mean ± SD from three independent experiments using triplicate measurements and statistically analyzed with Student’s t -test in each experiment. * p < 0.05, ** p < 0.01.

Article Snippet: Details of transwell migration assay was described previously , 2 × 10 4 cells in 200 µl of serum-free medium were added to the top chamber of the transwell (8-μm pore size, BD Biosciences, New Jersey, USA).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining, Migration, Colony Assay, CCK-8 Assay, Wound Healing Assay